Application
Western blot analysis of CBLB Antibody (Center) in HL-60 cell line lysates (35ug/lane). CBLB (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with CBLB Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
CBLB Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CBLB Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CBLB; RNF56; E3 ubiquitin-protein ligase CBL-B; Casitas B-lineage lymphoma proto-oncogene b; RING finger protein 56; SH3-binding protein CBL-B; Signal transduction protein CBL-B |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RNF56 |
|---|---|
| Alternative Names | CBLB RNF56 E3 ubiquitin-protein ligase CBL-B Casitas B-lineage lymphoma proto-oncogene b RING finger protein 56 SH3-binding protein CBL-B Signal transduction protein CBL-B |
| Molecular Weight(MW) | 109450 Da |
| Function | E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B- cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. Also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T- cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. May also be involved in EGFR ubiquitination and internalization |
| Tissue Specificity | Expressed in placenta, heart, lung, kidney, spleen, ovary and testis, as well as fetal brain and liver and hematopoietic cell lines, but not in adult brain, liver, pancreas, salivary gland, or skeletal muscle. Present in lymphocytes (at protein level) |
| Cellular Localization | Cytoplasm. Note=Upon EGF stimulation, associates with endocytic vesicles |
| Entrez Gene | 868 |
|---|
Application
Western blot analysis of CBLB Antibody (Center) in HL-60 cell line lysates (35ug/lane). CBLB (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with CBLB Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
CBLB Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
