Application
Western blot analysis of CBX1 Antibody (C-term) in MCF-7, A2058, Y79 cell line lysates (35ug/lane). CBX1 (arrow) was detected using the purified Pab.Application
CBX1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CBX1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
CBX1 Antibody (C-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CBX1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CBX1; CBX; Chromobox protein homolog 1; HP1Hsbeta; Heterochromatin protein 1 homolog beta; Heterochromatin protein p25; M31; Modifier 1 protein; p25beta |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CBX |
|---|---|
| Alternative Names | CBX1 CBX Chromobox protein homolog 1 HP1Hsbeta Heterochromatin protein 1 homolog beta Heterochromatin protein p25 M31 Modifier 1 protein p25beta |
| Molecular Weight(MW) | 21418 Da |
| Function | Component of heterochromatin. Recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Interaction with lamin B receptor (LBR) can contribute to the association of the heterochromatin with the inner nuclear membrane |
| Tissue Specificity | Expressed in all adult and embryonic tissues. |
| Cellular Localization | Nucleus. Note=Unassociated with chromosomes during mitosis |
| Entrez Gene | 10951 |
|---|
Application
Western blot analysis of CBX1 Antibody (C-term) in MCF-7, A2058, Y79 cell line lysates (35ug/lane). CBX1 (arrow) was detected using the purified Pab.
Application
CBX1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CBX1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
CBX1 Antibody (C-term) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
