Application
CCDC99 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the CCDC99 antibody detected the CCDC99 protein (arrow).Application
CCDC99 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CCDC99 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | CCDC99 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CCDC99 |
|---|---|
| Alternative Names | CCDC99 Protein Spindly Arsenite-related gene 1 protein Coiled-coil domain-containing protein 99 Rhabdomyosarcoma antigen MU-RMS-40.4A |
| Molecular Weight(MW) | 70172 Da |
| Function | Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment |
| Tissue Specificity | This CCDC99 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 458-488 amino acids from the C-terminal region of human CCDC99. |
| Cellular Localization | Cytoplasm, cytoskeleton, centrosome. Chromosome, centromere, kinetochore. Nucleus. Cytoplasm, cytoskeleton, spindle pole. Note=Localizes to the nucleus in interphase and to the kinetochore in early prometaphase Relocalizes to the mitotic spindle pole before metaphase and is subsequently lost from the spindle poles after chromosome congression is completed. Removal of this protein from the kinetochore requires the dynein/dynactin complex |
| Entrez Gene | 54908 |
|---|---|
| Nucleotide Accession | NP_060255.3 |
Application
CCDC99 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the CCDC99 antibody detected the CCDC99 protein (arrow).
Application
CCDC99 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CCDC99 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
