Application
Western blot analysis of CCNB1IP1 Antibody (C-term) in NCI-H460 cell line lysates (35ug/lane). CCNB1IP1 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with EED Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
CCNB1IP1 Antibody (C-term) flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CCNB1IP1 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | C14orf18, HEI10 |
|---|---|
| Alternative Names | CCNB1IP1 C14orf18 HEI10 E3 ubiquitin-protein ligase CCNB1IP1 Cyclin-B1-interacting protein 1 Human enhancer of invasion 10 |
| Molecular Weight(MW) | 31544 Da |
| Function | E3 ubiquitin-protein ligase. Modulates cyclin B levels and participates in the regulation of cell cycle progression through the G2 phase. Overexpression causes delayed entry into mitosis |
| Tissue Specificity | Highly expressed in heart. Detected at intermediate levels in liver and kidney, and at low levels in placenta, brain and lung |
| Cellular Localization | Nucleus. Chromosome. Note=May associate with segregating chromosomes during metaphase and anaphase |
| Entrez Gene | 57820 |
|---|
Application
Western blot analysis of CCNB1IP1 Antibody (C-term) in NCI-H460 cell line lysates (35ug/lane). CCNB1IP1 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with EED Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
CCNB1IP1 Antibody (C-term) flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
