Application
CCNH Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CCNH antibody detected the CCNH protein (arrow).Application
CCNH Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CCNH Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CCNH Cyclin-H MO15-associated protein p34 p37 |
|---|---|
| Molecular Weight(MW) | 37643 Da |
| Function | Regulates CDK7, the catalytic subunit of the CDK- activating kinase (CAK) enzymatic complex. CAK activates the cyclin-associated kinases CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive C-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle |
| Tissue Specificity | This CCNH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 267-299 amino acids from the C-terminal region of human CCNH. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 902 |
|---|---|
| Nucleotide Accession | NP_001230.1 |
Application
CCNH Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CCNH antibody detected the CCNH protein (arrow).
Application
CCNH Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
