Application
Western blot analysis of CCT3 Antibody (Center) in MCF7,CEM,K562,HL-60,Hela cell line lysates (35ug/lane).CCT3 (arrow) was detected using the purified Pab.Application
Western blot analysis of CCT3 (arrow) using rabbit polyclonal CCT3 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CCT3 gene (Lane 2) .Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CCT3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of K562 cells using CCT3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CCT3 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CCTG, TRIC5 |
|---|---|
| Alternative Names | CCT3 CCTG TRIC5 T-complex protein 1 subunit gamma CCT-gamma hTRiC5 |
| Molecular Weight(MW) | 60534 Da |
| Function | Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin |
| Tissue Specificity | This CCT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 305~335 amino acids from the Center region of human CCT3. |
| Cellular Localization | Cytoplasm. |
| Entrez Gene | 7203 |
|---|
Application
Western blot analysis of CCT3 Antibody (Center) in MCF7,CEM,K562,HL-60,Hela cell line lysates (35ug/lane).CCT3 (arrow) was detected using the purified Pab.
Application
Western blot analysis of CCT3 (arrow) using rabbit polyclonal CCT3 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CCT3 gene (Lane 2) .
Application
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with CCT3 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of K562 cells using CCT3 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
