Application
Western blot analysis of CD14(arrow) using rabbit polyclonal CD14 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD14 gene (Lane 2) (Origene Technologies).Application
CD14 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the CD14 antibody detected the CD14 protein (arrow).Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CD14 antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
CD14 Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CD14 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CD14 Monocyte differentiation antigen CD14 Myeloid cell-specific leucine-rich glycoprotein CD_antigen=CD14 Monocyte differentiation antigen CD14 urinary form Monocyte differentiation antigen CD14 membrane-bound form |
|---|---|
| Molecular Weight(MW) | 40076 Da |
| Function | In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules |
| Tissue Specificity | Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages |
| Cellular Localization | Cell membrane; Lipid-anchor, GPI-anchor. |
| Entrez Gene | 929 |
|---|
Application
Western blot analysis of CD14(arrow) using rabbit polyclonal CD14 Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD14 gene (Lane 2) (Origene Technologies).
Application
CD14 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the CD14 antibody detected the CD14 protein (arrow).
Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CD14 antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
CD14 Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
