Application
A. Western blot analysis on (A) A375, (B) fetal artery and (C) HUVEC cell lysates using anti-CD146 RabMAb (Cat. #AJ1152a), dilution 1:50,000.Application
B. Immunohistochemical analysis of paraffin-embedded melanoma using anti-CD146 RabMAb (Cat. #AJ1152a).| Product Name | CD146 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR3208 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MUC18 |
|---|---|
| Alternative Names | MCAM MUC18 Cell surface glycoprotein MUC18 Melanoma-associated antigen MUC18 Melanoma cell adhesion molecule Melanoma-associated antigen A32 S-endo 1 endothelial-associated antigen Cell surface glycoprotein P1H12 |
| Molecular Weight(MW) | 71607 Da |
| Function | Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration |
| Tissue Specificity | Detected in endothelial cells in vascular tissue throughout the body. May appear at the surface of neural crest cells during their embryonic migration. Appears to be limited to vascular smooth muscle in normal adult tissues Associated with tumor progression and the development of metastasis in human malignant melanoma. Expressed most strongly on metastatic lesions and advanced primary tumors and is only rarely detected in benign melanocytic nevi and thin primary melanomas with a low probability of metastasis |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
| Entrez Gene | 4162 |
|---|
Application
A. Western blot analysis on (A) A375, (B) fetal artery and (C) HUVEC cell lysates using anti-CD146 RabMAb (Cat. #AJ1152a), dilution 1:50,000.
Application
B. Immunohistochemical analysis of paraffin-embedded melanoma using anti-CD146 RabMAb (Cat. #AJ1152a).| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
