Application
CD1E Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the CD1E antibody detected the CD1E protein (arrow).Application
CD1E Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CD1E Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
CD1E Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of CD1E Antibody (Center)(Cat#AP9942c) with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Product Name | CD1E Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFCIF |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CD1E T-cell surface glycoprotein CD1e membrane-associated R2G1 CD_antigen=CD1e T-cell surface glycoprotein CD1e soluble |
|---|---|
| Molecular Weight(MW) | 43626 Da |
| Function | T-cell surface glycoprotein CD1e, soluble is required for the presentation of glycolipid antigens on the cell surface The membrane-associated form is not active |
| Tissue Specificity | Expressed on cortical thymocytes, dendritic cells, Langerhans cells, on certain T-cell leukemias, and in various other tissues |
| Cellular Localization | T-cell surface glycoprotein CD1e, membrane- associated: Golgi apparatus membrane; Single-pass type I membrane protein. Early endosome. Late endosome. Note=Predominantly localized in the trans-Golgi network in immature dendritic cells, and as a cleaved, soluble protein in the lysosome lumen of mature dendritic cells |
| Entrez Gene | 913 |
|---|---|
| Nucleotide Accession | NP_112155.2 |
Application
CD1E Antibody (Center) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the CD1E antibody detected the CD1E protein (arrow).
Application
CD1E Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human spleen followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CD1E Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
CD1E Antibody (Center) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of CD1E Antibody (Center)(Cat#AP9942c) with MDA-MB435 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
