Application
A. Western blot analysis on Raji cell lysate using anti-Phospho-CD22 (pY842) RabMAb (Cat. #AJ1157e), 1:2,000 dilution. Cells were either (A) untreated (B) treated with Pervanadate| Product Name | CD22 Antibody Phospho (pY842) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification | p-Y842 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | Y507 |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:2000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SIGLEC2 |
|---|---|
| Alternative Names | CD22 SIGLEC2 B-cell receptor CD22 Leu-14 B-lymphocyte cell adhesion molecule |
| Molecular Weight(MW) | 95348 Da |
| Function | Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules |
| Tissue Specificity | B-lymphocytes. |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein |
| Entrez Gene | 933 |
|---|
Application
A. Western blot analysis on Raji cell lysate using anti-Phospho-CD22 (pY842) RabMAb (Cat. #AJ1157e), 1:2,000 dilution. Cells were either (A) untreated (B) treated with Pervanadate| Application Notes | WB:1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
