Application
CD22 Antibody (Center) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the CD22 antibody detected the CD22 protein (arrow).Application
CD22 Antibody (Center) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of CD22 Antibody (Center)(Cat#AP10233c) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Product Name | CD22 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFCIF |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | SIGLEC2 |
|---|---|
| Alternative Names | CD22 SIGLEC2 B-cell receptor CD22 B-lymphocyte cell adhesion molecule Sialic acid-binding Ig-like lectin 2 T-cell surface antigen Leu-14 CD_antigen=CD22 Flags: Precursor |
| Molecular Weight(MW) | 95348 Da |
| Function | Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules |
| Tissue Specificity | B-lymphocytes. |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein |
| Entrez Gene | 933 |
|---|---|
| Nucleotide Accession | NP_001762.2 |
Application
CD22 Antibody (Center) western blot analysis in A375 cell line lysates (35ug/lane).This demonstrates the CD22 antibody detected the CD22 protein (arrow).
Application
CD22 Antibody (Center) flow cytometric analysis of A375 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of CD22 Antibody (Center)(Cat#AP10233c) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
