Application
Western blot analysis of CD28 Antibody (C-term) in MDA-MB231 cell line lysates (35ug/lane). CD28 (arrow) was detected using the purified Pab.Application
Confocal immunofluorescent analysis of CD28 Antibody (C-term) with Hela cell followed by Alexa Fluor® 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | CD28 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD28; T-cell-specific surface glycoprotein CD28; TP44; CD_antigen=CD28; Flags: Precursor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CD28 T-cell-specific surface glycoprotein CD28 TP44 CD_antigen=CD28 Flags: Precursor |
|---|---|
| Molecular Weight(MW) | 25066 Da |
| Function | Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival |
| Tissue Specificity | Expressed in T-cells and plasma cells, but not in less mature B-cells |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
| Entrez Gene | 940 |
|---|
Application
Western blot analysis of CD28 Antibody (C-term) in MDA-MB231 cell line lysates (35ug/lane). CD28 (arrow) was detected using the purified Pab.
Application
Confocal immunofluorescent analysis of CD28 Antibody (C-term) with Hela cell followed by Alexa Fluor® 489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
