Application
Western blot analysis on (A) THP-1 and (B) Jurkat cell lysates using anti-CD31 RabMAb , dilution 1:20,000.Application
Immunohistochemical analysis of paraffin-embedded human muscle using anti-CD31 RabMAb. Note positive staining of endothelial cells| Product Name | CD31/PECAM-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PECAM1, Platelet endothelial cell adhesion molecule, PECAM-1;EndoCAM;GPIIA' |
| Conjugation | Agarose |
| Modification | Acetylated |
| Clonality | Monoclonal |
|---|---|
| Isotype | Cocktail |
| Clone Number | EPR3094 |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | PECAM1 Platelet endothelial cell adhesion molecule PECAM-1 EndoCAM GPIIA' |
|---|---|
| Molecular Weight(MW) | 82536 Da |
| Function | Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC) |
| Tissue Specificity | Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U-937 histiocytic lymphoma cell lines (at protein level) |
| Cellular Localization | Isoform Long: Membrane; Single-pass type I membrane protein. Cell junction. Note=Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells |
| Entrez Gene | 5175 |
|---|
Application
Western blot analysis on (A) THP-1 and (B) Jurkat cell lysates using anti-CD31 RabMAb , dilution 1:20,000.
Application
Immunohistochemical analysis of paraffin-embedded human muscle using anti-CD31 RabMAb. Note positive staining of endothelial cells| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
