Application
Western blot analysis of CD38 antibody (C-term) (Cat.# AP2878b) in HepG2 cell line lysates (35ug/lane). CD38 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with CD38 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Immunofluorescence analysis of CD38 Antibody (C-term) with paraffin-embedded human hepatocarcinoma tissue . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.Application
Flow cytometric analysis of HepG2 cells using CD38 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of CD38 (arrow) using rabbit polyclonal CD38 Antibody (C-term) (Cat.# AP2878b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CD38 gene.Application
Confocal immunofluorescent analysis of CD38 Antibody (C-term) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Product Name | CD38 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD38; ADP-ribosyl cyclase 1; Cyclic ADP-ribose hydrolase 1; T10; CD_antigen=CD38 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CD38 ADP-ribosyl cyclase 1 Cyclic ADP-ribose hydrolase 1 T10 CD_antigen=CD38 |
|---|---|
| Molecular Weight(MW) | 34328 Da |
| Function | Synthesizes cyclic ADP-ribose, a second messenger for glucose-induced insulin secretion. Also has cADPr hydrolase activity. Also moonlights as a receptor in cells of the immune system |
| Tissue Specificity | Expressed at high levels in pancreas, liver, kidney, brain, testis, ovary, placenta, malignant lymphoma and neuroblastoma |
| Cellular Localization | Membrane; Single-pass type II membrane protein |
| Entrez Gene | 952 |
|---|
Application
Western blot analysis of CD38 antibody (C-term) (Cat.# AP2878b) in HepG2 cell line lysates (35ug/lane). CD38 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with CD38 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Immunofluorescence analysis of CD38 Antibody (C-term) with paraffin-embedded human hepatocarcinoma tissue . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
Application
Flow cytometric analysis of HepG2 cells using CD38 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of CD38 (arrow) using rabbit polyclonal CD38 Antibody (C-term) (Cat.# AP2878b). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CD38 gene.
Application
Confocal immunofluorescent analysis of CD38 Antibody (C-term) with Hela cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
