Application
Western blot analysis of CD4 Antibody (N-term) in CEM cell line lysates (35ug/lane). CD4(arrow) was detected using the purified Pab (1:60 dilution).Application
Western blot analysis of CD4 (arrow) using rabbit polyclonal CD4 Antibody (N-term) (RB12862). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD4 gene (Lane 2) (Origene Technologies).Application
CD4 Antibody (N-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CD4 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD4; T-cell surface glycoprotein CD4; T-cell surface antigen T4/Leu-3; CD_antigen=CD4; Flags: Precursor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CD4 T-cell surface glycoprotein CD4 T-cell surface antigen T4/Leu-3 CD_antigen=CD4 Flags: Precursor |
|---|---|
| Molecular Weight(MW) | 51111 Da |
| Function | Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts |
| Tissue Specificity | This CD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 63~93 amino acids from the N-terminal region of human CD4. |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein. Note=Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum |
| Entrez Gene | 920 |
|---|
Application
Western blot analysis of CD4 Antibody (N-term) in CEM cell line lysates (35ug/lane). CD4(arrow) was detected using the purified Pab (1:60 dilution).
Application
Western blot analysis of CD4 (arrow) using rabbit polyclonal CD4 Antibody (N-term) (RB12862). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CD4 gene (Lane 2) (Origene Technologies).
Application
CD4 Antibody (N-term) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. Then the antibody is purified by peptide affinity purification. |
