Application
Western blot analysis of CD54 antibody (C-term) (Cat.# AP2877b) in SK-Br-3 cell line lysates (35ug/lane). CD54 (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human lung tissue reacted with CD54 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of NCI-H292 cells using CD54 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CD54 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ICAM1; Intercellular adhesion molecule 1; Major group rhinovirus receptor; CD_antigen=CD54; Flags: Precursor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | ICAM1 Intercellular adhesion molecule 1 Major group rhinovirus receptor CD_antigen=CD54 Flags: Precursor |
|---|---|
| Molecular Weight(MW) | 57825 Da |
| Function | ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans- endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation In case of rhinovirus infection acts as a cellular receptor for the virus |
| Tissue Specificity | This CD54 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 495~524 amino acids from the C-terminal region of human CD54. |
| Cellular Localization | Membrane; Single-pass type I membrane protein |
| Entrez Gene | 3383 |
|---|
Application
Western blot analysis of CD54 antibody (C-term) (Cat.# AP2877b) in SK-Br-3 cell line lysates (35ug/lane). CD54 (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human lung tissue reacted with CD54 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of NCI-H292 cells using CD54 Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
