Application
CD63 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the CD63 antibody detected the CD63 protein (arrow).Application
CD63 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CD63 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
CD63 Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CD63 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD63; MLA1; TSPAN30; CD63 antigen; Granulophysin; Lysosomal-associated membrane protein 3; Melanoma-associated antigen ME491; OMA81H; Ocular melanoma-associated antigen; Tetraspanin-30; CD_antigen=CD63 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MLA1 TSPAN30 |
|---|---|
| Alternative Names | CD63 MLA1 TSPAN30 CD63 antigen Granulophysin Lysosomal-associated membrane protein 3 Melanoma-associated antigen ME491 OMA81H Ocular melanoma-associated antigen Tetraspanin-30 CD_antigen=CD63 |
| Molecular Weight(MW) | 25637 Da |
| Function | This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation |
| Tissue Specificity | Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Note=Also found in Weibel- Palade bodies of endothelial cells. Located in platelet dense granules |
| Entrez Gene | 967 |
|---|---|
| Nucleotide Accession | NP_001771.1 |
Application
CD63 Antibody (C-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the CD63 antibody detected the CD63 protein (arrow).
Application
CD63 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skin carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CD63 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
CD63 Antibody (C-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
