Application
IHC image using - Detection of CD63 by IHC in human melanoma| Product Name | CD63 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [NK1 / C3] to CD63CD63 is expressed on activated platelets. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. It is also present in monocytes and macrophages and is weakly expressed on granulocytes, B, and T cells. CD63 is identical to the melanoma-associated antigen which isME491 and to the platelet antigen PTLGP40. |
| Immunogen | Smooth plasma membrane fraction of MeWo cells. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | NK1/C3 |
| Host Species | Mouse |
| Tested Applications | IHCIHC-P |
| This antibody may be diluted to a titer of 1:100 to 1:200 in an ABC method. High temperature antigen unmasking with 10mM citrate buffer, pH 6.0 is required prior to immunostaining. We suggest an incubation period of 30 minutes at room temperature. Optimal dilutions / concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | TSPAN30 967 ME491 CD63 P08962 155740 LAMP-3 LAMP3 MLA1 OMA81H LAMP 3 |
|---|---|
| Target | CD63 |
| Tissue Specificity | This antibody is specific to a 53 kD protein, expressed on activated platelets. CD63 is a lysosomal membrane glycoprotein that is translocated to plasma membrane after platelet activation. It is also expressed on granulocytes, B cells and T cells. |
| Cellular Localization | Cytoplasmic. |
| Light Chain Type | kappa |
Application
IHC image using - Detection of CD63 by IHC in human melanoma| Application Notes | This antibody may be diluted to a titer of 1:100 to 1:200 in an ABC method. High temperature antigen unmasking with 10mM citrate buffer, pH 6.0 is required prior to immunostaining. We suggest an incubation period of 30 minutes at room temperature. Optimal dilutions / concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | PBS, pH7.2 with 0.02% sodium azide as preservative |
