Application
Western blot analysis of CD68 Antibody (N-term) in HL-60 cell line lysates (35ug/lane). CD68 (arrow) was detected using the purified Pab.Application
CD68 Antibody (N-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CD68 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD68; Macrosialin; Gp110; CD_antigen=CD68; Flags: Precursor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100-500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CD68 Macrosialin Gp110 CD_antigen=CD68 Flags: Precursor |
|---|---|
| Molecular Weight(MW) | 37408 Da |
| Function | Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells |
| Tissue Specificity | Highly expressed by blood monocytes and tissue macrophages. Also expressed in lymphocytes, fibroblasts and endothelial cells. Expressed in many tumor cell lines which could allow them to attach to selectins on vascular endothelium, facilitating their dissemination to secondary sites |
| Cellular Localization | Isoform Short: Cell membrane; Single-pass type I membrane protein |
| Entrez Gene | 968 |
|---|
Application
Western blot analysis of CD68 Antibody (N-term) in HL-60 cell line lysates (35ug/lane). CD68 (arrow) was detected using the purified Pab.
Application
CD68 Antibody (N-term) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100-500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
