Application
Western blot analysis of CD73 Pab in mouse brain tissue lysate. CD73 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.Application
Western blot analysis of NT5E (arrow) using rabbit polyclonal NT5E Antibody (M535) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NT5E gene.Application
Formalin-fixed and paraffin-embedded human Placenta tissue reacted with CD73 (NT5E) Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | CD73 (NT5E) Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NT5E; NT5; NTE; 5'-nucleotidase; Ecto-5'-nucleotidase; CD_antigen=CD73; Flags: Precursor |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | NT5 NTE |
|---|---|
| Alternative Names | NT5E NT5 NTE 5'-nucleotidase Ecto-5'-nucleotidase CD_antigen=CD73 Flags: Precursor |
| Molecular Weight(MW) | 63368 Da |
| Function | Hydrolyzes extracellular nucleotides into membrane permeable nucleosides |
| Tissue Specificity | This CD73 (NT5E) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 528~557 amino acids from the C-terminal region of human NT5E. |
| Cellular Localization | Cell membrane; Lipid-anchor, GPI-anchor. |
Application
Western blot analysis of CD73 Pab in mouse brain tissue lysate. CD73 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Application
Western blot analysis of NT5E (arrow) using rabbit polyclonal NT5E Antibody (M535) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the NT5E gene.
Application
Formalin-fixed and paraffin-embedded human Placenta tissue reacted with CD73 (NT5E) Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
