Application
His6-cdc14 Antibody western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the cdc14 antibody detected the cdc14 protein (arrow).Application
Cdc14 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Cdc14 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.Application
Cdc14 Antibody flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | Cdc14 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDC14A; Dual specificity protein phosphatase CDC14A; CDC14 cell division cycle 14 homolog A |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CDC14A Dual specificity protein phosphatase CDC14A CDC14 cell division cycle 14 homolog A |
|---|---|
| Molecular Weight(MW) | 66574 Da |
| Function | Dual-specificity phosphatase. Required for centrosome separation and productive cytokinesis during cell division. May dephosphorylate the APC subunit FZR1/CDH1, thereby promoting APC- FZR1 dependent degradation of mitotic cyclins and subsequent exit from mitosis |
| Tissue Specificity | This Cdc14 antibody is generated from rabbits immunized with a recombinant protein encoding aa 1~379 of human cdc14. |
| Cellular Localization | Nucleus. Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle. Note=Centrosomal during interphase, released into the cytoplasm at the onset of mitosis. Subsequently localizes to the midzone of the mitotic spindle |
Application
His6-cdc14 Antibody western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the cdc14 antibody detected the cdc14 protein (arrow).
Application
Cdc14 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Cdc14 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Cdc14 Antibody flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
