Application
Western blot analysis of CDC14B Antibody (Center) in 293 cell line lysates (35ug/lane). CDC14B (arrow) was detected using the purified Pab.Application
CDC14B Antibody (Center) flow cytometry analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CDC14B Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDC14B; Dual specificity protein phosphatase CDC14B; CDC14 cell division cycle 14 homolog B |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CDC14B Dual specificity protein phosphatase CDC14B CDC14 cell division cycle 14 homolog B |
|---|---|
| Molecular Weight(MW) | 56802 Da |
| Function | Dual-specificity phosphatase involved in DNA damage response. Essential regulator of the G2 DNA damage checkpoint: following DNA damage, translocates to the nucleus and dephosphorylates FZR1/CDH1, a key activator of the anaphase promoting complex/cyclosome (APC/C). Dephosphorylation of FZR1/CDH1 activates the APC/C, leading to the ubiquitination of PLK1, preventing entry into mitosis. Preferentially dephosphorylates proteins modified by proline-directed kinases |
| Tissue Specificity | This CDC14B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 271~301 amino acids from the Center region of human CDC14B. |
| Cellular Localization | Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Following DNA damage, translocates from the nucleolus to the nucleoplasm and interacts with FZR1/CDH1 |
| Entrez Gene | 8555 |
|---|
Application
Western blot analysis of CDC14B Antibody (Center) in 293 cell line lysates (35ug/lane). CDC14B (arrow) was detected using the purified Pab.
Application
CDC14B Antibody (Center) flow cytometry analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
