Application
Western blot analysis of Cdc25A Pab(Cat# AP6272c) in Hela cell line lysates (35ug/lane). Cdc25A (arrow) was detected using the purified Pab (1:60 dilution).Application
Formalin-fixed and paraffin-embedded human Spleen tissue reacted with Cdc25A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | Cdc25A Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDC25A; M-phase inducer phosphatase 1; Dual specificity phosphatase Cdc25A |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CDC25A M-phase inducer phosphatase 1 Dual specificity phosphatase Cdc25A |
|---|---|
| Molecular Weight(MW) | 59087 Da |
| Function | Tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. Directly dephosphorylates CDK1 and stimulates its kinase activity. Also dephosphorylates CDK2 in complex with cyclin E, in vitro |
| Tissue Specificity | This Cdc25A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human Cdc25A. |
| Entrez Gene | 993 |
|---|
Application
Western blot analysis of Cdc25A Pab(Cat# AP6272c) in Hela cell line lysates (35ug/lane). Cdc25A (arrow) was detected using the purified Pab (1:60 dilution).
Application
Formalin-fixed and paraffin-embedded human Spleen tissue reacted with Cdc25A Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
