Application
A. Western blot analysis on HeLa cell lysate using anti-Cdc25C RabMAb (Cat. #AJ1175a); dilution 1:5,000.Application
B. Immunohistochemical analysis of paraffin-embedded human urinary bladder carcinoma using anti- Cdc25C RabMAb (Cat. #AJ1175a).| Product Name | Cdc25C Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDC25C, M-phase inducer phosphatase 3, Dual specificity phosphatase Cdc25C |
| Clonality | Monoclonal |
|---|---|
| Clone Number | E302 |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:1000~10000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CDC25C M-phase inducer phosphatase 3 Dual specificity phosphatase Cdc25C |
|---|---|
| Molecular Weight(MW) | 53365 Da |
| Function | Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity |
| Tissue Specificity | A synthetic peptide corresponding to residues near N-terminus of human Cdc25C was used as immunogen. The antibody can also detect splice isoform 2, 4 and 5 of human Cdc25C, based on sequence homology. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 995 |
|---|
Application
A. Western blot analysis on HeLa cell lysate using anti-Cdc25C RabMAb (Cat. #AJ1175a); dilution 1:5,000.
Application
B. Immunohistochemical analysis of paraffin-embedded human urinary bladder carcinoma using anti- Cdc25C RabMAb (Cat. #AJ1175a).| Application Notes | WB:1:1000~10000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
