Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-Cdc25C (pS216) RabMAb (Cat. #AJ1175c); dilution 1:1,000. Cells were (1) untreated and (2) treated with Phosphatase.| Product Name | Cdc25C Antibody Phospho (pS216) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDC25C, M-phase inducer phosphatase 3, Dual specificity phosphatase Cdc25C |
| Modification | p-S216 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | E190 |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CDC25C M-phase inducer phosphatase 3 Dual specificity phosphatase Cdc25C |
|---|---|
| Molecular Weight(MW) | 53365 Da |
| Function | Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity |
| Tissue Specificity | A synthetic phospho-peptide corresponding to residues surrounding Ser216 of human Cdc25C was used as immunogen. The antibody only detects Cdc25C phosphorylated on Serine 216. |
| Cellular Localization | Nucleus. |
| Entrez Gene | 995 |
|---|
Application
A. Western blot analysis on HeLa cell lysates using anti-Phospho-Cdc25C (pS216) RabMAb (Cat. #AJ1175c); dilution 1:1,000. Cells were (1) untreated and (2) treated with Phosphatase.| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
