Application
Western blot analysis of CDCA8 Antibody (Center) in HL60 cell line lysates (35ug/lane). CDCA8(arrow) was detected using the purified polyclonal antibody.Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CDCA8 antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Flow cytometric analysis of HL-60 cells using CDCA8 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CDCA8 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDCA8; PESCRG3; Borealin; Dasra-B; Cell division cycle-associated protein 8; Pluripotent embryonic stem cell-related gene 3 protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PESCRG3 |
|---|---|
| Alternative Names | CDCA8 PESCRG3 Borealin Dasra-B Cell division cycle-associated protein 8 Pluripotent embryonic stem cell-related gene 3 protein |
| Molecular Weight(MW) | 31323 Da |
| Function | Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly In the complex, it may be required to direct the CPC to centromeric DNA. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment |
| Tissue Specificity | This CDCA8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 122~152 amino acids from the center region of human CDCA8. |
| Cellular Localization | Nucleus, nucleolus. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Chromosome, centromere. Note=Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with SENP3 in the nucleolus in interphase cells |
| Entrez Gene | 55143 |
|---|
Application
Western blot analysis of CDCA8 Antibody (Center) in HL60 cell line lysates (35ug/lane). CDCA8(arrow) was detected using the purified polyclonal antibody.
Application
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CDCA8 antibody (Center) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Flow cytometric analysis of HL-60 cells using CDCA8 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
