Application
Western blot analysis of anti-CDH4 Antibody (N-term) (RB13654) in HepG2 cell line lysates (35ug/lane). CDH4(arrow) was detected using the purified Pab (1:60 dilution).Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with CDH4 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Immunofluorescence analysis of CDH4 Antibody (N-term) Antibody with paraffin-embedded human brain tissue . 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.Application
Flow cytometric analysis of HepG2 cells using CDH4 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CDH4 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDH4; Cadherin-4; Retinal cadherin |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCIFFC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CDH4 Cadherin-4 Retinal cadherin |
|---|---|
| Molecular Weight(MW) | 100281 Da |
| Function | Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. May play an important role in retinal development |
| Tissue Specificity | Expressed mainly in brain but also found in other tissues |
| Cellular Localization | Cell membrane; Single-pass type I membrane protein |
| Entrez Gene | 1002 |
|---|
Application
Western blot analysis of anti-CDH4 Antibody (N-term) (RB13654) in HepG2 cell line lysates (35ug/lane). CDH4(arrow) was detected using the purified Pab (1:60 dilution).
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with CDH4 antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Immunofluorescence analysis of CDH4 Antibody (N-term) Antibody with paraffin-embedded human brain tissue . 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.
Application
Flow cytometric analysis of HepG2 cells using CDH4 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. Then the antibody is purified by peptide affinity purification. |
