Application
Western blot analysis of CDK8 (arrow) using rabbit polyclonal CDK8 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CDK8 gene.Application
CDK8 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CDK8 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDK8; Cyclin-dependent kinase 8; Cell division protein kinase 8; Mediator complex subunit CDK8; Mediator of RNA polymerase II transcription subunit CDK8; Protein kinase K35 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:50~100 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CDK8 Cyclin-dependent kinase 8 Cell division protein kinase 8 Mediator complex subunit CDK8 Mediator of RNA polymerase II transcription subunit CDK8 Protein kinase K35 |
|---|---|
| Molecular Weight(MW) | 53284 Da |
| Function | Component of the Mediator complex, a coactivator involved in regulated gene transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Phosphorylates the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAp II), which may inhibit the formation of a transcription initiation complex Phosphorylates CCNH leading to down-regulation of the TFIIH complex and transcriptional repression. Recruited through interaction with MAML1 to hyperphosphorylate the intracellular domain of NOTCH, leading to its degradation |
| Tissue Specificity | This CDK8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 256-285 amino acids from the Central region of human CDK8. |
| Cellular Localization | Nucleus (Probable). |
Application
Western blot analysis of CDK8 (arrow) using rabbit polyclonal CDK8 Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CDK8 gene.
Application
CDK8 Antibody (Center) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:50~100 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
