Application
A. Western blot analysis on (A) HT-29 (B) HL-60 (C) HeLa and (D) 293 cell lysates using anti-CDK9 RabMAb (Cat. #AJ1180a), dilution 1:2000.Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-CDK9 RabMAb (Cat. #AJ1180a).| Product Name | CDK9 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDK9, CDC2L4, Cell division protein kinase 9, Cyclin-dependent kinase 9;Serine/threonine-protein kinase PITALRE;Cell division cycle 2-like protein kinase 4 |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EPR3119Y |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:2000~20000 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CDC2L4 TAK |
|---|---|
| Alternative Names | CDK9 CDC2L4 Cell division protein kinase 9 Cyclin-dependent kinase 9 Serine/threonine-protein kinase PITALRE Cell division cycle 2-like protein kinase 4 |
| Molecular Weight(MW) | 42778 Da |
| Function | Protein kinase involved in the regulation of transcription. Member of the cyclin-dependent kinase pair (CDK9/cyclin-T) complex, also called positive transcription elongation factor b (P-TEFb), which facilitates the transition from abortive to productive elongation by phosphorylating the CTD (C-terminal domain) of the large subunit of RNA polymerase II (RNAP II) POLR2A, SUPT5H and RDBP. This complex is inactive when in the 7SK snRNP complex form. Phosphorylates EP300, MYOD1, RPB1/POLR2A and AR, and the negative elongation factors DSIF and NELF. Regulates cytokine inducible transcription networks by facilitating promoter recognition of target transcription factors (e.g. TNF-inducible RELA/p65 activation and IL-6-inducible STAT3 signaling). Promotes RNA synthesis in genetic programs for cell growth, differentiation and viral pathogenesis. P-TEFb is also involved in cotranscriptional histone modification, mRNA processing and mRNA export. Modulates a complex network of chromatin modifications including histone H2B monoubiquitination (H2Bub1), H3 lysine 4 trimethylation (H3K4me3) and H3K36me3; integrates phosphorylation during transcription with chromatin modifications to control co-transcriptional histone mRNA processing. The CDK9/cyclin-K complex has also a kinase activity towards CTD of RNAP II and can substitute for CDK9/cyclin-T P-TEFb in vitro. Replication stress response protein; the CDK9/cyclin-K complex is required for genome integrity maintenance, by promoting cell cycle recovery from replication arrest and limiting single- stranded DNA amount in response to replication stress, thus reducing the breakdown of stalled replication forks and avoiding DNA damage. In addition, probable function in DNA repair of isoform 2 via interaction with KU70/XRCC6. Promotes cardiac myocyte enlargement. RPB1/POLR2A phosphorylation on 'Ser-2' in CTD activates transcription. AR phosphorylation modulates AR transcription factor promoter selectivity and cell growth. DSIF and NELF phosphorylation promotes transcription by inhibiting their negative effect. The phosphorylation of MYOD1 enhances its transcriptional activity and thus promotes muscle differentiation |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Nucleus. Cytoplasm. Nucleus, PML body. Note=Accumulates on chromatin in response to replication stress Complexed with CCNT1 in nuclear speckles, but uncomplexed form in the cytoplasm. The translocation from nucleus to cytoplasm is XPO1/CRM1-dependent. Associates with PML body when acetylated |
| Entrez Gene | 1025 |
|---|
Application
A. Western blot analysis on (A) HT-29 (B) HL-60 (C) HeLa and (D) 293 cell lysates using anti-CDK9 RabMAb (Cat. #AJ1180a), dilution 1:2000.
Application
B. Immunohistochemical analysis of paraffin-embedded human breast carcinoma using anti-CDK9 RabMAb (Cat. #AJ1180a).| Application Notes | WB:1:2000~20000 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
