Application
CENPE Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CENPE antibody detected the CENPE protein (arrow).| Product Name | CENPE Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CENPE; Centromere-associated protein E; Centromere protein E; Kinesin-related protein CENPE |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CENPE Centromere-associated protein E Centromere protein E Kinesin-related protein CENPE |
|---|---|
| Molecular Weight(MW) | 316415 Da |
| Function | Essential for the maintenance of chromosomal stability through efficient stabilization of microtubule capture at kinetochores. Plays a key role in the movement of chromosomes toward the metaphase plate during mitosis. Is a slow plus end- directed motor whose activity is essential for metaphase chromosome alignment. Couples chromosome position to microtubule depolymerizing activity. The highly processive microtubule- dependent motor activity of CENPE serves to power chromosome congression and provides a flexible, motile tether linking kinetochores to dynamic spindle microtubules. Necessary for the mitotic checkpoint signal at individual kinetochores to prevent aneuploidy due to single chromosome loss. Required for the efficient recruitment of BUBR1, MAD1 and MAD2 to attached and newly unattached kinetochores. Stimulates mammalian BUBR1 kinase activity. Accumulates just before mitosis at the G2 phase of the cell cycle |
| Tissue Specificity | This CENPE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2572-2602 amino acids from the C-terminal region of human CENPE. |
| Cellular Localization | Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Note=Associates with kinetochores during congression (as early as prometaphase), relocates to the spindle midzone at anaphase, and is quantitatively discarded at the end of the cell division |
Application
CENPE Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the CENPE antibody detected the CENPE protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
