Application
Western blot analysis of CENPK Antibody (C-term) in mouse liver tissue lysates (35ug/lane). CENPK (arrow) was detected using the purified Pab.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with CENPK Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | CENPK Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CENPK; ICEN37; Centromere protein K; Interphase centromere complex protein 37; Protein AF-5alpha; p33 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | ICEN37 |
|---|---|
| Alternative Names | CENPK ICEN37 Centromere protein K Interphase centromere complex protein 37 Protein AF-5alpha p33 |
| Molecular Weight(MW) | 31655 Da |
| Function | Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Acts in coordination with CASC5/KNL1 to recruit the NDC80 complex to the outer kinetochore |
| Tissue Specificity | Detected in several fetal organs with highest levels in fetal liver. In adults, it is weakly expressed in lung and placenta |
| Cellular Localization | Nucleus. Chromosome, centromere. Chromosome, centromere, kinetochore. Note=Localizes exclusively in the centromeres. The CENPA-CAD complex is probably recruited on centromeres by the CENPA-NAC complex |
| Entrez Gene | 64105 |
|---|
Application
Western blot analysis of CENPK Antibody (C-term) in mouse liver tissue lysates (35ug/lane). CENPK (arrow) was detected using the purified Pab.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with CENPK Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
