Application
A. Western blot analysis on HeLa cell lysate using anti-CENP-A RabMAb (Cat. #AJ1182a), dilution 1:20,000Application
B. Immunofluorescent staining of HeLa cells using anti-CENP-A RabMAb (Cat. #AJ1182a).| Product Name | CENP-A Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CENPA, Histone H3-like centromeric protein A, Centromere autoantigen A |
| Clonality | Monoclonal |
|---|---|
| Clone Number | EP800Y |
| Host Species | Rabbit |
| Tested Applications | WBIF |
| WB:1:100000~200000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CENPA Histone H3-like centromeric protein A Centromere autoantigen A |
|---|---|
| Molecular Weight(MW) | 15991 Da |
| Function | Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division. The CENPA-H4 heterotetramer can bind DNA by itself (in vitro) |
| Tissue Specificity | A synthetic peptide corresponding to residues in the C-term of human CENP-A was used as an immunogen. |
| Cellular Localization | Nucleus. Chromosome, centromere, kinetochore. Note=Localizes exclusively in the kinetochore domain of centromeres. Occupies a compact domain at the inner kinetochore plate stretching across 2 thirds of the length of the constriction but encompassing only one third of the constriction width and height |
| Entrez Gene | 1058 |
|---|
Application
A. Western blot analysis on HeLa cell lysate using anti-CENP-A RabMAb (Cat. #AJ1182a), dilution 1:20,000
Application
B. Immunofluorescent staining of HeLa cells using anti-CENP-A RabMAb (Cat. #AJ1182a).| Application Notes | WB:1:100000~200000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
