Application
CENPA Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the CENPA antibody detected the CENPA protein (arrow).| Product Name | CENPA Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CENPA; Histone H3-like centromeric protein A; Centromere autoantigen A; Centromere protein A |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CENPA Histone H3-like centromeric protein A Centromere autoantigen A Centromere protein A |
|---|---|
| Molecular Weight(MW) | 15991 Da |
| Function | Histone H3-like variant which exclusively replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore. Required for recruitment and assembly of kinetochore proteins, mitotic progression and chromosome segregation. May serve as an epigenetic mark that propagates centromere identity through replication and cell division. The CENPA-H4 heterotetramer can bind DNA by itself (in vitro) |
| Tissue Specificity | This CENPA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-110 amino acids from the Central region of human CENPA. |
| Cellular Localization | Nucleus. Chromosome, centromere, kinetochore. Note=Localizes exclusively in the kinetochore domain of centromeres. Occupies a compact domain at the inner kinetochore plate stretching across 2 thirds of the length of the constriction but encompassing only one third of the constriction width and height |
| Entrez Gene | 1058 |
|---|---|
| Nucleotide Accession | NP_001800.1 NP_001035891.1 |
Application
CENPA Antibody (Center) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the CENPA antibody detected the CENPA protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
