Application
Western blot analysis of CENPM Antibody (C-term) in Ramos cell line lysates (35ug/lane). CENPM (arrow) was detected using the purified Pab.Application
Western blot analysis of CENPM Antibody (C-term) in mouse NIH-3T3 cell line lysates (35ug/lane). CENPM (arrow) was detected using the purified Pab.Application
CENPM Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CENPM Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
CENPM Antibody (C-term) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CENPM Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CENPM; C22orf18; ICEN39; PANE1; Centromere protein M; Interphase centromere complex protein 39; Proliferation-associated nuclear element protein 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | C22orf18 ICEN39 PANE1 |
|---|---|
| Alternative Names | CENPM C22orf18 ICEN39 PANE1 Centromere protein M Interphase centromere complex protein 39 Proliferation-associated nuclear element protein 1 |
| Molecular Weight(MW) | 19737 Da |
| Function | Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres |
| Tissue Specificity | Isoform 3 is highly expressed in spleen, and intermediately in heart, prostate and ovary. Isoform 3 is highly expressed in resting CD19 B-cells and B-lineage chronic lymphocytic leukemia (B-CLL) cells and weakly expressed in activated B-cells. Isoform 1 is selectively expressed in activated CD19 cells and weakly in resting CD19 B-cells |
| Cellular Localization | Nucleus. Cytoplasm. Chromosome, centromere, kinetochore. Note=Nuclear in non-confluent cells and cytoplasmic in confluent or dividing cells (By similarity). Localizes in the kinetochore domain of centromeres |
| Entrez Gene | 79019 |
|---|
Application
Western blot analysis of CENPM Antibody (C-term) in Ramos cell line lysates (35ug/lane). CENPM (arrow) was detected using the purified Pab.
Application
Western blot analysis of CENPM Antibody (C-term) in mouse NIH-3T3 cell line lysates (35ug/lane). CENPM (arrow) was detected using the purified Pab.
Application
CENPM Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CENPM Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
CENPM Antibody (C-term) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
