Application
Western blot analysis of CENPQ Antibody (Center) in Hela cell line lysates (35ug/lane).CENPQ (arrow) was detected using the purified Pab.Application
CENPQ Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CENPQ Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.Application
CENPQ Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CENPQ Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CENPQ; C6orf139; Centromere protein Q |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | C6orf139 |
|---|---|
| Alternative Names | CENPQ C6orf139 Centromere protein Q |
| Molecular Weight(MW) | 30595 Da |
| Function | Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. May be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex |
| Tissue Specificity | This CENPQ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 135-165 amino acids from the Central region of human CENPQ. |
| Cellular Localization | Nucleus. Chromosome, centromere. Note=Localizes exclusively in the centromeres. The CENPA-CAD complex is probably recruited on centromeres by the CENPA-NAC complex |
| Entrez Gene | 55166 |
|---|
Application
Western blot analysis of CENPQ Antibody (Center) in Hela cell line lysates (35ug/lane).CENPQ (arrow) was detected using the purified Pab.
Application
CENPQ Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CENPQ Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
CENPQ Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
