Application
The anti-CERK Pab is used in Western blot to detect CERK in mouse heart tissue lysate (Lane 1) and A2058 cell lysate (Lane 2).| Product Name | CERK Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CERK; KIAA1646; Ceramide kinase; Acylsphingosine kinase; Lipid kinase 4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | KIAA1646 |
|---|---|
| Alternative Names | CERK KIAA1646 Ceramide kinase Acylsphingosine kinase Lipid kinase 4 |
| Molecular Weight(MW) | 59977 Da |
| Function | Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. Acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines. Binds phosphoinositides |
| Tissue Specificity | High level expression in heart, brain, skeletal muscle, kidney and liver; moderate in peripheral blood leukocytes and thymus; very low in spleen, small intestine, placenta and lung |
| Cellular Localization | Cytoplasm. Membrane; Peripheral membrane protein |
Application
The anti-CERK Pab is used in Western blot to detect CERK in mouse heart tissue lysate (Lane 1) and A2058 cell lysate (Lane 2).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
