Application
Western blot analysis of CHAF1B Antibody (C-term) in A2058 cell line lysates (35ug/lane). CHAF1B (arrow) was detected using the purified Pab.Application
CHAF1B Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CHAF1B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Product Name | CHAF1B Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CHAF1B; CAF1A; CAF1P60; MPHOSPH7; MPP7; Chromatin assembly factor 1 subunit B; Chromatin assembly factor I p60 subunit; M-phase phosphoprotein 7 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CAF1A CAF1P60 MPHOSPH7 MPP7 |
|---|---|
| Alternative Names | CHAF1B CAF1A CAF1P60 MPHOSPH7 MPP7 Chromatin assembly factor 1 subunit B Chromatin assembly factor I p60 subunit M-phase phosphoprotein 7 |
| Molecular Weight(MW) | 61493 Da |
| Function | Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. The CCR4-NOT complex functions as general transcription regulation complex |
| Tissue Specificity | This CHAF1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human CHAF1B. |
| Cellular Localization | Nucleus. Cytoplasm. Note=DNA replication foci. Cytoplasmic in M phase |
| Entrez Gene | 8208 |
|---|
Application
Western blot analysis of CHAF1B Antibody (C-term) in A2058 cell line lysates (35ug/lane). CHAF1B (arrow) was detected using the purified Pab.
Application
CHAF1B Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CHAF1B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
