| Product Name | CAF1A antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse anti-Chromatin Assembly Factor 1 (1SS-48) monoclonal antibodyChromatin assembly factor-1 (CAF-1) is a multi protein complex that functions to assemble nucleosomes in a replication-dependent fashion. The CAF-1 complex is composed of three subunits, p150, p60 and p48 in stoichiometric amounts. The composition of CAF-1 is conserved among human Drosophila and yeast. Human CAF-1 deposits newly synthesized, acetylated histones onto replicated DNA where it localizes in S-phase cells. Localization of the CAF-1 complex to the newly synthesized DNA is mediated in part by PCNA which functions to "mark" the appropriate places for loading of CAF-1 and in fact, PCNA may be co-precipitated with CAF-1. During G1, G2, and S-phases, p150/p60 is found in a complex with a subfraction of p48 in the nucleus. During mitosis there is a hyperphos-phorylation of the p60 subunit and a corresponding enrichment in the cytosolic fraction of the p150 and p60 proteins. While characteristically a nuclear protein there is variation in level of staining intensity between cells and with the use of different fixatives. Although no disease states have been definitively identified as having alterations in expression of any of the CAF-1 subunits, the p150 subunit has been shown to map to 21q22.2, a region strongly associated with the major feature of Downs Syndrome. |
| Immunogen | Mice were immunized with human CAF-1 p130 subunit protein and fusing the splenocytes with P3-X63-Ag8-653 mouse myeloma cells. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1SS-48 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIPWB |
| IF (2.5 ug/ml), IP 2.5 ug/sample), Western blot (2.5 ug/ml): |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CAF1P60 601245 MPP7 CHAF1B MPHOSPH7 CAF1A CAF1 Q13112 CAF-1 CAFIP60 8208 CAF-IP60 CAF 1 CAF IP60 |
|---|---|
| Target | CAF1A |
| Application Notes | IF (2.5 ug/ml), IP 2.5 ug/sample), Western blot (2.5 ug/ml): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20° C. Avoid multiple freeze/thaw cycles. |
| Storage Buffer | Contains 50% glycerol, 0.01% sodium azide and 1.0 mg/ml BSA. |
