Application
Western blot analysis of CHAT Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane).CHAT (arrow) was detected using the purified Pab.Application
Western blot analysis of CHAT Antibody (N-term) in mouse liver tissue lysates (35ug/lane). CHAT (arrow) was detected using the purified Pab.Application
Confocal immunofluorescent analysis of CHAT Antibody (N-term) with NCI-H460 cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).IF
(A) MNs [choline acetyltransferase (ChAT)-positive cells, red] and astrocytes [glial fibrillary acidic protein (GFAP)-positive cells, green] expression in four groups for three stages. (B) MNs (ChAT-positive cells, red) and microcytes (Iba1-positive cells, green) expression in four groups for three stages. Scale bars represent 100 µm.| Product Name | CHAT Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description |
|
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFWB |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CHAT Choline O-acetyltransferase |
|---|---|
| Molecular Weight(MW) | 82536 Da |
| Function | Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses |
| Tissue Specificity | This CHAT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 106~135 amino acids from the N-terminal region of human CHAT. |
| Entrez Gene | 1103 |
|---|
Application
Western blot analysis of CHAT Antibody (N-term) in NCI-H460 cell line lysates (35ug/lane).CHAT (arrow) was detected using the purified Pab.
Application
Western blot analysis of CHAT Antibody (N-term) in mouse liver tissue lysates (35ug/lane). CHAT (arrow) was detected using the purified Pab.
Application
Confocal immunofluorescent analysis of CHAT Antibody (N-term) with NCI-H460 cell followed by Alexa Fluor搴?488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
IF
(A) MNs [choline acetyltransferase (ChAT)-positive cells, red] and astrocytes [glial fibrillary acidic protein (GFAP)-positive cells, green] expression in four groups for three stages. (B) MNs (ChAT-positive cells, red) and microcytes (Iba1-positive cells, green) expression in four groups for three stages. Scale bars represent 100 µm.| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
