Application
CHFR Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the CHFR antibody detected the CHFR protein (arrow).| Product Name | CHFR Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CHFR; RNF196; E3 ubiquitin-protein ligase CHFR; Checkpoint with forkhead and RING finger domains protein; RING finger protein 196 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | RNF196 |
|---|---|
| Alternative Names | CHFR RNF196 E3 ubiquitin-protein ligase CHFR Checkpoint with forkhead and RING finger domains protein RING finger protein 196 |
| Molecular Weight(MW) | 73386 Da |
| Function | E3 ubiquitin-protein ligase that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. Acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. Probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Promotes the ubiquitination and subsequent degradation of AURKA and PLK1 Probably acts as a tumor suppressor, possibly by mediating the polyubiquitination of HDAC1, leading to its degradation. May also promote the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress |
| Tissue Specificity | Ubiquitous. |
| Cellular Localization | Nucleus, PML body. |
| Entrez Gene | 55743 |
|---|---|
| Nucleotide Accession | NP_001154818.1 NP_001154817.1 |
Application
CHFR Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the CHFR antibody detected the CHFR protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
