Application
CHMP1A Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the CHMP1A antibody detected the CHMP1A protein (arrow).| Product Name | CHMP1A Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CHMP1A; CHMP1; KIAA0047; PCOLN3; PRSM1; Charged multivesicular body protein 1a; Chromatin-modifying protein 1a; Vacuolar protein sorting-associated protein 46-1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CHMP1 KIAA0047 PCOLN3 PRSM1 |
|---|---|
| Alternative Names | CHMP1A CHMP1 KIAA0047 PCOLN3 PRSM1 Charged multivesicular body protein 1a Chromatin-modifying protein 1a Vacuolar protein sorting-associated protein 46-1 |
| Molecular Weight(MW) | 21703 Da |
| Function | Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT- III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in cytokinesis. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. May also be involved in chromosome condensation. Targets the Polycomb group (PcG) protein BMI1/PCGF4 to regions of condensed chromatin. May play a role in stable cell cycle progression and in PcG gene silencing |
| Tissue Specificity | Expressed in placenta, cultured skin fibroblasts and in osteoblast cell line MG-63 |
| Cellular Localization | Cytoplasm. Endosome membrane; Peripheral membrane protein. Nucleus matrix. Note=The cytoplasmic form is partially membrane-associated and localizes to early endosomes The nuclear form remains associated with the chromosome scaffold during mitosis. On overexpression, it localizes to nuclear bodies characterized by nuclease-resistant condensed chromatin |
| Entrez Gene | 5119 |
|---|---|
| Nucleotide Accession | NP_002759.2 |
Application
CHMP1A Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the CHMP1A antibody detected the CHMP1A protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
