Application
CHMP1B Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CHMP1B antibody detected the CHMP1B protein (arrow).Application
CHMP1B Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CHMP1B Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
CHMP1B Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CHMP1B Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CHMP1B; C18orf2; Charged multivesicular body protein 1b; CHMP1.5; Chromatin-modifying protein 1b; Vacuolar protein sorting-associated protein 46-2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | C18orf2 |
|---|---|
| Alternative Names | CHMP1B C18orf2 Charged multivesicular body protein 1b CHMP1.5 Chromatin-modifying protein 1b Vacuolar protein sorting-associated protein 46-2 |
| Molecular Weight(MW) | 22109 Da |
| Function | Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT- III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in cytokinesis. Involved in recruiting VPS4A and/or VPS4B and SPAST to the midbody of dividing cells. Involved in HIV-1 p6- and p9-dependent virus release |
| Tissue Specificity | Widely expressed. Expressed in pancreas, kidney, skeletal muscle, liver, lung, placenta and brain |
| Cellular Localization | Cytoplasm, cytosol. Endosome. Late endosome membrane; Peripheral membrane protein (Probable). Note=Localizes to the midbody of dividing cells, colocalizing with CEP55 and CHMP5. Localized at the periphery of the Fleming body |
| Entrez Gene | 57132 |
|---|---|
| Nucleotide Accession | NP_065145.2 |
Application
CHMP1B Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the CHMP1B antibody detected the CHMP1B protein (arrow).
Application
CHMP1B Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CHMP1B Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
CHMP1B Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
