Application
CHMP5 Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the CHMP5 antibody detected the CHMP5 protein (arrow).| Product Name | CHMP5 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CHMP5; C9orf83; SNF7DC2; Charged multivesicular body protein 5; Chromatin-modifying protein 5; SNF7 domain-containing protein 2; Vacuolar protein sorting-associated protein 60 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | C9orf83 SNF7DC2 |
|---|---|
| Alternative Names | CHMP5 C9orf83 SNF7DC2 Charged multivesicular body protein 5 Chromatin-modifying protein 5 SNF7 domain-containing protein 2 Vacuolar protein sorting-associated protein 60 |
| Molecular Weight(MW) | 24571 Da |
| Function | Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT- III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in HIV-1 p6- and p9-dependent virus release |
| Tissue Specificity | This CHMP5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 177-204 amino acids from the C-terminal region of human CHMP5. |
| Cellular Localization | Cytoplasm, cytosol. Endosome membrane; Peripheral membrane protein (Probable). Note=Localizes to the midbody of dividing cells. Localized in two distinct rings on either side of the Fleming body |
| Entrez Gene | 51510 |
|---|---|
| Nucleotide Accession | NP_057494.3 |
Application
CHMP5 Antibody (C-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the CHMP5 antibody detected the CHMP5 protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
