Application
CHST2 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the CHST2 antibody detected the CHST2 protein (arrow).Application
CHST2 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CHST2 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CHST2; GN6ST; Carbohydrate sulfotransferase 2; Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 2; N-acetylglucosamine 6-O-sulfotransferase 1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | GN6ST |
|---|---|
| Alternative Names | CHST2 GN6ST Carbohydrate sulfotransferase 2 Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 2 N-acetylglucosamine 6-O-sulfotransferase 1 |
| Molecular Weight(MW) | 57857 Da |
| Function | Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues within keratan-like structures on N-linked glycans and within mucin-associated glycans that can ultimately serve as SELL ligands. SELL ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Participates in biosynthesis of the SELL ligand sialyl 6-sulfo Lewis X and in lymphocyte homing to Peyer patches Has no activity toward O-linked sugars. Its substrate specificity may be influenced by its subcellular location. Sulfates GlcNAc residues at terminal, non-reducing ends of oligosaccharide chains |
| Tissue Specificity | Widely expressed. Highly expressed in bone marrow, peripheral blood leukocytes, spleen, brain, spinal cord, ovary and placenta. Expressed by high endothelial cells (HEVs) and leukocytes |
| Cellular Localization | Golgi apparatus, trans-Golgi network membrane; Single-pass type II membrane protein (Probable) |
| Entrez Gene | 9435 |
|---|---|
| Nucleotide Accession | NP_004258.2 |
Application
CHST2 Antibody (Center) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the CHST2 antibody detected the CHST2 protein (arrow).
Application
CHST2 Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
