Application
Western blot analysis of CHST7 Antibody (Center) in CEM cell line lysates (35ug/lane). CHST7 (arrow) was detected using the purified Pab.Application
CHST7 Antibody (Center) flow cytometric analysis of U251 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CHST7 Antibody (Center) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CHST7; Carbohydrate sulfotransferase 7; Chondroitin 6-sulfotransferase 2; Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 5; N-acetylglucosamine 6-O-sulfotransferase 4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBFC |
| WB:1:100~500 FC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CHST7 Carbohydrate sulfotransferase 7 Chondroitin 6-sulfotransferase 2 Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 5 N-acetylglucosamine 6-O-sulfotransferase 4 |
|---|---|
| Molecular Weight(MW) | 54266 Da |
| Function | Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of non-reducing N-acetylglucosamine (GlcNAc) residues. Preferentially acts on mannose-linked GlcNAc. Also able to catalyze the transfer of sulfate to position 6 of the N- acetylgalactosamine (GalNAc) residue of chondroitin. Also acts on core 2 mucin-type oligosaccharide and N-acetyllactosamine oligomer with a lower efficiency. Has weak or no activity toward keratan sulfate and oligosaccharides containing the Galbeta1-4GlcNAc Catalyzes 6-O-sulfation of beta-benzyl GlcNAc but not alpha- or beta-benzyl GalNAc |
| Tissue Specificity | Widely expressed. Highly expressed in heart, spleen, liver and ovary. Expressed at lower level in brain, placenta, thyroid, spinal cord and peripheral blood leukocytes Not expressed in adult skin |
| Cellular Localization | Golgi apparatus membrane; Single-pass type II membrane protein (By similarity) |
| Entrez Gene | 56548 |
|---|
Application
Western blot analysis of CHST7 Antibody (Center) in CEM cell line lysates (35ug/lane). CHST7 (arrow) was detected using the purified Pab.
Application
CHST7 Antibody (Center) flow cytometric analysis of U251 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 FC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
