Application
CIDEA Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the CIDEA antibody detected the CIDEA protein (arrow).| Product Name | CIDEA Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CIDEA; Cell death activator CIDE-A; Cell death-inducing DFFA-like effector A |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:100~500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CIDEA Cell death activator CIDE-A Cell death-inducing DFFA-like effector A |
|---|---|
| Molecular Weight(MW) | 24687 Da |
| Function | Acts as a CEBPB coactivator in mammary epithelial cells to control the expression of a subset of CEBPB downstream target genes, including ID2, IGF1, PRLR, SOCS1, SOCS3, XDH, but not casein. By interacting with CEBPB, strengthens the association of CEBPB with the XDH promoter, increases histone acetylation and dissociates HDAC1 from the promoter (By similarity). Binds to lipid droplets and regulates their enlargement, thereby restricting lipolysis and favoring storage. At focal contact sites between lipid droplets, promotes directional net neutral lipid transfer from the smaller to larger lipid droplets. The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair and occurs at a lower rate than that promoted by CIDEC. When overexpressed, induces apoptosis. The physiological significance of its role in apoptosis is unclear |
| Tissue Specificity | Expressed in omental and subcutaneous adipose tissue (at protein level) |
| Cellular Localization | Lipid droplet. Nucleus (By similarity). Note=Enriched at lipid droplet contact sites. Has been shown to localize to mitonchondria, where it could interact with UCP1 and hence inhibit UCP1 uncoupling activity (By similarity). These data could not be confirmed (PubMed:18509062) |
| Entrez Gene | 1149 |
|---|---|
| Nucleotide Accession | NP_001270.1 |
Application
CIDEA Antibody (C-term) western blot analysis in CEM cell line lysates (35ug/lane).This demonstrates the CIDEA antibody detected the CIDEA protein (arrow).| Application Notes | WB:1:100~500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
