Application
Western blot analysis of CJ119 Antibody (N-term) in MDA-MB231 cell line lysates (35ug/lane). CJ119 (arrow) was detected using the purified Pab.Application
CJ119 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CJ119 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
CJ119 Antibody (N-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CJ119 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MCMBP; C10orf119; Mini-chromosome maintenance complex-binding protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | C10orf119 |
|---|---|
| Alternative Names | MCMBP C10orf119 Mini-chromosome maintenance complex-binding protein |
| Molecular Weight(MW) | 72980 Da |
| Function | Associated component of the MCM complex that acts as a regulator of DNA replication. Binds to the MCM complex during late S phase and promotes the disassembly of the MCM complex from chromatin, thereby acting as a key regulator of pre-replication complex (pre-RC) unloading from replicated DNA. Can dissociate the MCM complex without addition of ATP; probably acts by destabilizing interactions of each individual subunits of the MCM complex. Required for sister chromatid cohesion |
| Tissue Specificity | This CJ119 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 150~179 amino acids from the N-terminal region of human CJ119. |
| Cellular Localization | Nucleus. Note=Associates with chromatin. Highly associated with chromatin in G1/S and S phases, reduced binding to chromatin in G2, and further decreased binding in early M phase. It then reassociates with chromatin in late M phase Dissociates from chromatin later than component of the MCM complex |
| Entrez Gene | 79892 |
|---|
Application
Western blot analysis of CJ119 Antibody (N-term) in MDA-MB231 cell line lysates (35ug/lane). CJ119 (arrow) was detected using the purified Pab.
Application
CJ119 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human cervix carcinoma tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CJ119 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
CJ119 Antibody (N-term) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
