Application
Western blot of chicken brain tissue incubated with CLC4 Antibody (C-term) (Cat.# AP6329f). Data courtesy of Emily McMains, Louisiana State University.Application
Immunofluorescence image of cultured chick retinal amacrine (neuronal) cells labeled with CLC4 Antibody (C-term) (Cat # AP6329f). Data courtesy of Emily McMains, Louisiana State University.Application
Retinae were collected from adult White Leghorn chicken and fixed in the eyecup for two hours in 4% Paraformaldehyde (in PBS). Retinae were then removed from the eyecups and incubated in 30% sucrose (in PBS) overnight. Retinal tissue was embedded and frozen in OCT compound and cut into ~15um sections on a cryotome. Sections were blocked in 5% normal goat serum (in 1%BSA/.1%saponin PBS) for one hour and then incubated at RT with 1:250 (1%BSA/.1% saponin PBS) ClC4 antibody (Cat.# AP6329f) for 1 hour. Sections were then washed in PBS (3X10minutes) and then treated with secondary antibody (1:500 Cy3) for one hour. After another PBS wash series, sections were coverslipped and antibody labeling was visualized at 20X with a Leica upright microscope using a TRITC filter set and Xenon lamp illumination. (Crousillac et al, 2003)| Product Name | CLC4 Antibody (C-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CLCN4; H(+)/Cl(-) exchange transporter 4; Chloride channel protein 4; Chloride transporter ClC-4 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIFIHC |
| WB:1:100~500 IF |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CLCN4 H(+)/Cl(-) exchange transporter 4 Chloride channel protein 4 Chloride transporter ClC-4 |
|---|---|
| Molecular Weight(MW) | 84917 Da |
| Function | Proton-coupled chloride transporter. Functions as antiport system and exchanges chloride ions against protons |
| Tissue Specificity | Abundant in skeletal muscle and also detectable in brain and heart |
| Cellular Localization | Early endosome membrane; Multi-pass membrane protein (By similarity). Late endosome membrane; Multi-pass membrane protein (By similarity) |
| Entrez Gene | 1183 |
|---|
Application
Western blot of chicken brain tissue incubated with CLC4 Antibody (C-term) (Cat.# AP6329f). Data courtesy of Emily McMains, Louisiana State University.
Application
Immunofluorescence image of cultured chick retinal amacrine (neuronal) cells labeled with CLC4 Antibody (C-term) (Cat # AP6329f). Data courtesy of Emily McMains, Louisiana State University.
Application
Retinae were collected from adult White Leghorn chicken and fixed in the eyecup for two hours in 4% Paraformaldehyde (in PBS). Retinae were then removed from the eyecups and incubated in 30% sucrose (in PBS) overnight. Retinal tissue was embedded and frozen in OCT compound and cut into ~15um sections on a cryotome. Sections were blocked in 5% normal goat serum (in 1%BSA/.1%saponin PBS) for one hour and then incubated at RT with 1:250 (1%BSA/.1% saponin PBS) ClC4 antibody (Cat.# AP6329f) for 1 hour. Sections were then washed in PBS (3X10minutes) and then treated with secondary antibody (1:500 Cy3) for one hour. After another PBS wash series, sections were coverslipped and antibody labeling was visualized at 20X with a Leica upright microscope using a TRITC filter set and Xenon lamp illumination. (Crousillac et al, 2003)| Application Notes | WB:1:100~500 IF |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
