Application
Western blot analysis of CLDN2 (arrow) using rabbit polyclonal CLDN2 Antibody (Y224) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CLDN2 gene.Application
CLDN2 Antibody (C-term Y224) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CLDN2 Antibody (C-term Y224) for immunohistochemistry. Clinical relevance has not been evaluated.Application
CLDN2 Antibody (C-term Y224) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CLDN2 Antibody (C-term Y224) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CLDN2; Claudin-2; SP82 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Alternative Names | CLDN2 Claudin-2 SP82 |
|---|---|
| Molecular Weight(MW) | 24549 Da |
| Function | Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity (By similarity) |
| Tissue Specificity | This CLDN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 207-230 amino acids from the C-terminal region of human CLDN2. |
| Cellular Localization | Cell junction, tight junction (By similarity). Cell membrane; Multi-pass membrane protein (By similarity) |
| Entrez Gene | 9075 |
|---|---|
| Nucleotide Accession | NP_001164566.1 NP_001164563.1 |
Application
Western blot analysis of CLDN2 (arrow) using rabbit polyclonal CLDN2 Antibody (Y224) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CLDN2 gene.
Application
CLDN2 Antibody (C-term Y224) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CLDN2 Antibody (C-term Y224) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
CLDN2 Antibody (C-term Y224) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
