Application
CLDN16 Antibody (N-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the CLDN16 antibody detected the CLDN16 protein (arrow).Application
CLDN16 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human Kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CLDN16 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
CLDN16 Antibody (N-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | CLDN16 Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CLDN16; PCLN1; Claudin-16; Paracellin-1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHCFC |
| WB:1:100~500 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PCLN1 |
|---|---|
| Alternative Names | CLDN16 PCLN1 Claudin-16 Paracellin-1 |
| Molecular Weight(MW) | 33836 Da |
| Function | Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. Involved in paracellular magnesium reabsorption. Required for a selective paracellular conductance. May form, alone or in partnership with other constituents, an intercellular pore permitting paracellular passage of magnesium and calcium ions down their electrochemical gradients. Alternatively, it could be a sensor of magnesium concentration that could alter paracellular permeability mediated by other factors |
| Tissue Specificity | Kidney-specific, including the thick ascending limb of Henle (TAL) |
| Cellular Localization | Cell junction, tight junction. Cell membrane; Multi-pass membrane protein |
| Entrez Gene | 10686 |
|---|---|
| Nucleotide Accession | NP_006571.1 |
Application
CLDN16 Antibody (N-term) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the CLDN16 antibody detected the CLDN16 protein (arrow).
Application
CLDN16 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human Kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CLDN16 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
CLDN16 Antibody (N-term) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | WB:1:100~500 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
